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Alpha-gal - dvě strany jedné mince
ŠABATKOVÁ, Klára
Ticks are interesting ectoparasites for their ability to modulate the host's defense response. This ability of tick saliva is often exploited by pathogens to increase their transmission. Therefore many researchers focused on identification of molecules in tick saliva and their possible medical use. This thesis focuses on the high natural presence of anti-Gal antibodies in human serum and it's interaction with alpha-gal epitope that is expressed on the surface of some pathogens and mammalian cells. This epitope has been identified in tick saliva and some drugs as well.
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Measurement of signalling pathway activation in mouse macrophage line IC-21 and primery dendritic cells after infection with tick-borne encephalitis virus.
Kožantová, Jana ; Lieskovská, Jaroslava (advisor) ; Drbal, Karel (referee)
Tick-borne encephalitis is a serious disease of the central nervous system. It is caused by tick-borne encephalitis virus, which is transmitted by ticks. The Czech Republic is one of the countries with the highest prevalence of this disease. Tick-borne encephalitis virus is able to replicate in several cell types. In this work we focused on macrophage line IC-21 and dendritic cells, because these cells are the first, which encounter the virus and support its spreading in the host at early stage of infection. So far there is not known any specific receptor for virus entry into cells or which signaling pathways activates. Therefore, we decided to investigate the activation of selected signaling pathways after infection with tick-borne encephalitis virus and influence of tick saliva on this activation. We employed methods of dual luciferase reporter assay, immunosandwich assay and western blot. The obtained results showed that in virus infected IC-21 cells are activated phosphatidyl-inositol pathway, NF-κB pathway, signaling molecule Erk1/2 and others. Testing of tick saliva effect revealed significantly decreased activity of NF-κB, AP-1 and CREB.
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